Skill UI

Provide a comprehensive toolkit for RNA velocity inference in single-cell RNA-seq data, modeling unspliced/spliced kinetics to infer differentiation directions, latent time, and driver genes while complementing Scanpy pipelines.

AnnData is a specialized Python package designed for managing and analyzing annotated matrices, particularly crucial in single-cell genomics and bioinformatics. It efficiently stores experimental measurements (X) alongside comprehensive metadata (obs, var, obsm, etc.). This skill allows users to read, write, subset, and concatenate large, complex datasets in formats like .h5ad and .zarr, making it essential for scRNA-seq analysis workflows and integration with the scverse ecosystem.

scVelo is a powerful Python package designed for RNA velocity analysis using single-cell RNA-seq data. It estimates cell state transitions by modeling the kinetics of mRNA splicing—specifically using the ratio of unspliced to spliced mRNA abundances. This allows researchers to reconstruct developmental trajectories and predict cell fates, even when only snapshot data is available, making it a vital complement to standard trajectory inference tools like Scanpy.