Skill UI

This comprehensive skill provides a standardized pipeline for reconstructing the evolutionary history of biological sequences (genes, proteins, genomes). It guides users through essential steps, including multiple sequence alignment using MAFFT, maximum likelihood tree inference with IQ-TREE 2, and visualization with ETE3. Ideal for research in viral phylodynamics, molecular clock studies, and large-scale comparative genomics.

Pysam is a comprehensive Python toolkit for handling genomic data. It provides a Pythonic interface for reading, writing, and manipulating essential genomic formats, including SAM/BAM/CRAM alignments, VCF/BCF variants, and FASTA/FASTQ sequences. It is critical for developing robust bioinformatics pipelines, performing coverage analysis, and analyzing genetic variations.

scikit-bio is a comprehensive Python library designed for advanced bioinformatics and biological data analysis. It provides robust capabilities for sequence manipulation (DNA, RNA, Protein), performing complex sequence alignments (pairwise and multiple), constructing and analyzing phylogenetic trees, and calculating diverse ecological metrics. It is essential for microbiome analysis, evolutionary studies, and general biological data processing, supporting standard formats like FASTA, Newick, and BIOM.

This skill runs a comprehensive, sequenced pipeline (Patterns 1-5) to structurally analyze a monolithic codebase. It inventories components, detects domain duplication, resolves hierarchy issues, measures inter-module coupling, and groups components into candidate, domain-aligned services. It is essential for architects planning service extraction, providing actionable metrics and DDD insights before refactoring or splitting the application.